Hot stuff (aka. sticky Sxy)
After a grueling few days of bandshift experiments to study CRP and Sxy binding to the pilA promoter, we have made the excellent discovery that Sxy binds to DNA. Surprisingly, it appears that Sxy may bind to the CRP site in this promoter. We have several promoter constructs that lack various parts of the wildtype promoter - we can use these to directly test whether CRP and Sxy bind to the same site. We are taking a break from bandshifts this afternoon to analyze the data and plan our next set of experiments. We will begin to frame these results as a figure for our DNA-binding manuscript.