4/4 is even better
Today I received the sequencing results from my second putative clone of the ICAP-9G,14C variant and it was good! Thus, I now have all of my ICAP variants and so can proceed with more bandshift analysis, while at the same time conducting CRP-Sxy bandshifts along with Sunita.
Further analysis of the first ICAP-9G,14C clone revealed that it lacked a restriction site where the sequencing primer was targeted to bind, consistent with the failed sequencing reaction that I described in my last post. That plasmid is now in the trash and I can file the experience away as another one of those funny little cloning complications.